Description
These tracks show the RNA-Seq coverage for different D. ananassae tissues. The RNA-Seq read coverage was normalized by a wigsum of
100,000,000 (1 million 100bp reads). The RNA-Seq reads were mapped against
the D. ananassae DanaCAF1 genome assembly using
HISAT2. RNA-Seq reads
from multiple replicates were combined together.
The read coverage tracks were produced by the bam2wig.py program in RSeQC.
The RNA-Seq data were retrieved from the NCBI Sequence Read Archive using the
BioProject accession numbers
PRJNA257286 and
PRJNA388952.
References
Yang H, Jaime M, Polihronakis M, Kanegawa K, Markow T, Kaneshiro K, Oliver B.
Re-annotation of eight Drosophila genomes.
Life Sci Alliance. 2018 Dec 24;1(6):e201800156.
Rogers RL, Shao L, Sanjak JS, Andolfatto P, Thornton KR.
Revised annotations, sex-biased expression, and lineage-specific genes in the Drosophila melanogaster group.
G3 (Bethesda). 2014 Oct 1;4(12):2345-51.
Kim D, Langmead B, Salzberg SL.
HISAT: a fast spliced aligner with low memory requirements.
Nat Methods. 2015 Apr;12(4):357-60.
Wang L, Wang S, Li W.
RSeQC: quality control of RNA-seq experiments.
Bioinformatics. 2012 Aug 15;28(16):2184-5.