Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DariGB1    Primary Table: DmelTranscriptsBLAT    Row Count: 27,865   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom LSRM01000001varchar(255) values
chromStart 62856int(10) unsigned range
chromEnd 66754int(10) unsigned range
name Coq8-RAvarchar(255) values
score 600int(10) unsigned range
strand -char(1) values
thickStart 62856int(10) unsigned range
thickEnd 66754int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 6int(11) range
blockSizes 553,782,57,105,60,141longblob  
chromStarts 0,1550,3232,3373,3619,3757longblob  
id FBtr0073697varchar(255) values
description FBgn0052649longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
LSRM010000016285666754Coq8-RA600-628566675406553,782,57,105,60,1410,1550,3232,3373,3619,3757FBtr0073697FBgn0052649
LSRM010000016815469447CG17486-RB288+68154694470563,129,147,153,720,522,807,987,1221FBtr0299924FBgn0032997
LSRM010000017082071498Flad1-RA480-70820714980368,171,3090,114,369FBtr0073695FBgn0030431
LSRM010000017082071498Flad1-RB480-70820714980368,171,3090,114,369FBtr0073696FBgn0030431
LSRM010000017093171486Flad2-RA356-709317148602166,420,513FBtr0080501FBgn0032522
LSRM010000017285475626CG4404-RA642-728547562604129,17,167,1540,814,918,2618FBtr0073694FBgn0030432
LSRM010000017682177268mRpL49-RA456+7682177268014470FBtr0073691FBgn0030433
LSRM010000017682177268mRpL49-RB456+7682177268014470FBtr0343185FBgn0030433
LSRM010000017835379196Brms1-RA644-783537919504257,238,78,920,326,646,751FBtr0073693FBgn0030434
LSRM010000017981681008CG4645-RA554+79816810080748,64,66,216,207,93,1800,59,159,285,577,860,1012FBtr0073692FBgn0030435

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. arizonae genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. arizonae genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.