Schema for LTRharvest - LTR Fragments Identified by LTRharvest
  Database: DbusGB1    Primary Table: ltrdigest_PBS    Row Count: 8   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 768smallint(5) unsigned range
chrom CP012523varchar(255) values
chromStart 24086101int(10) unsigned range
chromEnd 24086114int(10) unsigned range
name LTR_retrotransposon6_PBSvarchar(255) values
score 1000int(10) unsigned range
strand +char(1) values

Sample Rows
 
binchromchromStartchromEndnamescorestrand
768CP0125232408610124086114LTR_retrotransposon6_PBS1000+
636CP01252668051346805149LTR_retrotransposon37_PBS1000+
693CP0125261426565214265665LTR_retrotransposon53_PBS1000+
710CP0125261650780816507826LTR_retrotransposon55_PBS1000+
778CP0125262532042425320435LTR_retrotransposon61_PBS1000-
778CP0125262539553525395549LTR_retrotransposon63_PBS1000+
593CP01252710735251073539LTR_retrotransposon67_PBS1000+
700CP0125281518136315181380LTR_retrotransposon79_PBS1000+

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description
 

Description

LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. busckii genome using the following parameters:

-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best

Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters:

-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50

The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters:

-psmall 80 -plarge 30

References