Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DereCAF1    Primary Table: DmelTranscriptsBLAT    Row Count: 32,147   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom scaffold_1121varchar(255) values
chromStart 273int(10) unsigned range
chromEnd 414int(10) unsigned range
name His3:CG33815-RAvarchar(255) values
score 844int(10) unsigned range
strand -char(1) values
thickStart 273int(10) unsigned range
thickEnd 414int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 1int(11) range
blockSizes 141longblob  
chromStarts 0longblob  
id FBtr0091819varchar(255) values
description FBgn0053815longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
scaffold_1121273414His3:CG33815-RA844-273414011410FBtr0091819FBgn0053815
scaffold_1121273414His3:CG33818-RA844-273414011410FBtr0091822FBgn0053818
scaffold_1121273414His3:CG33803-RA844-273414011410FBtr0091807FBgn0053803
scaffold_1121273414His3:CG33821-RA844-273414011410FBtr0091825FBgn0053821
scaffold_1121273414His3:CG33824-RA844-273414011410FBtr0091828FBgn0053824
scaffold_1121273414His3:CG33812-RA844-273414011410FBtr0091816FBgn0053812
scaffold_1121273414His3:CG33827-RA844-273414011410FBtr0091831FBgn0053827
scaffold_1121273414His3:CG33830-RA844-273414011410FBtr0091834FBgn0053830
scaffold_1121273414His3:CG33833-RA844-273414011410FBtr0091837FBgn0053833
scaffold_1121273414His3:CG33836-RA844-273414011410FBtr0091840FBgn0053836

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. erecta genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. erecta genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.