Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DeugGB2    Primary Table: refseqRNA    Row Count: 23,815   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom AFPQ02000031varchar(255) values
chromStart 17537int(10) unsigned range
chromEnd 23978int(10) unsigned range
name XM_017210188varchar(255) values
score 1000int(10) unsigned range
strand +char(1) values
thickStart 18278int(10) unsigned range
thickEnd 23768int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 10int(10) unsigned range
blockSizes 449,254,160,138,491,478,271...longblob  
chromStarts 0,507,861,1162,1356,1902,37...longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585AFPQ020000311753723978XM_0172101881000+1827823768010449,254,160,138,491,478,271,155,291,321,0,507,861,1162,1356,1902,3730,5555,5772,6120,
585AFPQ020002070450XM_017229081990-2034002137,257,0,193,
585AFPQ0200021373912XM_017212907982-16083202261,266,0,573,
585AFPQ0200046729033786XM_041819793996-290337830279,797,0,86,
585AFPQ0200046742085401XR_005993643994+4208420803432,397,306,0,434,887,
585AFPQ0200046743415401XR_005993642998+4341434103299,397,306,0,301,754,
585AFPQ0200046749295401XM_0418177401000+4937530202110,306,0,166,
585AFPQ02000534355837XM_041819438990+20305837024360,208,55,34,271,68,11,13,138,55,32,3,224,607,7,137,429,86,1077,3,9,105,1232,287,0,372,581,637,999,1270,1338,1349,1362,1500,1559,1592,1596,1822,2429,2436,2573,3002,3088,4165,4168,4177,4282,5515,
585AFPQ020005349645837XM_041819668988+20305837018341,68,24,138,55,26,6,3,224,106,501,7,137,429,86,568,1231,287,0,341,409,433,571,630,656,663,667,893,999,1500,1507,1644,2073,2159,3354,4586,
585AFPQ0200053410415837XM_041817727984+20305837020264,68,11,13,121,17,55,25,224,607,7,137,429,86,1077,3,9,105,1232,287,0,264,332,343,356,477,494,553,590,816,1423,1430,1567,1996,2082,3159,3162,3171,3276,4509,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. eugracilis transcripts from the NCBI RefSeq database were aligned against the D. eugracilis assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. eugracilis transcripts were mapped against the D. eugracilis genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_018153835.1.