Schema for LTRharvest - LTR Fragments Identified by LTRharvest
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Database: DeugGB2 Primary Table: ltrdigest_LTR Row Count: 1,186   Data last updated: 2022-10-20
field | example | SQL type | info |
bin | 585 | smallint(5) unsigned | range |
chrom | AFPQ02000031 | varchar(255) | values |
chromStart | 26274 | int(10) unsigned | range |
chromEnd | 31275 | int(10) unsigned | range |
name | LTR_retrotransposon1_LTR | varchar(255) | values |
score | 1000 | int(10) unsigned | range |
strand | + | char(1) | values |
thickStart | 26274 | int(10) unsigned | range |
thickEnd | 31275 | int(10) unsigned | range |
reserved | 0 | int(10) unsigned | range |
blockCount | 2 | int(10) unsigned | range |
blockSizes | 255,265 | longblob | |
chromStarts | 0,4736 | longblob | |
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Sample Rows
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bin | chrom | chromStart | chromEnd | name | score | strand | thickStart | thickEnd | reserved | blockCount | blockSizes | chromStarts |
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585 | AFPQ02000031 | 26274 | 31275 | LTR_retrotransposon1_LTR | 1000 | + | 26274 | 31275 | 0 | 2 | 255,265 | 0,4736 |
585 | AFPQ02000031 | 35050 | 40718 | LTR_retrotransposon2_LTR | 1000 | - | 35050 | 40718 | 0 | 2 | 172,172 | 0,5496 |
585 | AFPQ02000176 | 8305 | 18619 | LTR_retrotransposon3_LTR | 1000 | + | 8305 | 18619 | 0 | 2 | 249,250 | 0,10064 |
585 | AFPQ02000204 | 834 | 5452 | LTR_retrotransposon4_LTR | 1000 | - | 834 | 5452 | 0 | 2 | 1440,1425 | 0,3193 |
585 | AFPQ02000231 | 4496 | 5690 | LTR_retrotransposon5_LTR | 1000 | - | 4496 | 5690 | 0 | 2 | 99,98 | 0,1096 |
585 | AFPQ02000231 | 21705 | 24882 | LTR_retrotransposon6_LTR | 1000 | - | 21705 | 24882 | 0 | 2 | 424,432 | 0,2745 |
585 | AFPQ02000290 | 355 | 8026 | LTR_retrotransposon7_LTR | 1000 | + | 355 | 8026 | 0 | 2 | 1318,1332 | 0,6339 |
585 | AFPQ02000290 | 18064 | 24976 | LTR_retrotransposon8_LTR | 1000 | + | 18064 | 24976 | 0 | 2 | 335,315 | 0,6597 |
585 | AFPQ02000294 | 8017 | 12403 | LTR_retrotransposon9_LTR | 1000 | - | 8017 | 12403 | 0 | 2 | 324,324 | 0,4062 |
585 | AFPQ02000303 | 5352 | 9748 | LTR_retrotransposon10_LTR | 1000 | + | 5352 | 9748 | 0 | 2 | 319,317 | 0,4079 |
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Note: all start coordinates in our database are 0-based, not
1-based. See explanation
here.
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LTRharvest (ltrdigest) Track Description
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Description
LTRharvest
is used to identify putative long terminal repeats (LTR)
retrotransposons in the D. eugracilis genome using the following parameters:
-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7
-mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best
Additional features within the LTR retrotransposons are annotated by
LTRdigest
with the following parameters:
-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30
-pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5
-pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6
-maxgaplen 50
The subset of candidates that show significant similarity to Pfam protein domains
within LTR retrotransposons are selected and then clustered using the ltrclustering program
with the following parameters:
-psmall 80 -plarge 30
References
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Ellinghaus D, Kurtz S, and Willhoeft U.
LTRharvest,
an efficient and flexible software for de novo detection of LTR retrotransposons.
BMC Bioinformatics, 9:18, 2008.
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Steinbiss S, Willhoeft U, Gremme G, and Kurtz S.
Fine-grained
annotation and classification of de novo predicted LTR retrotransposons.
Nucleic Acids Research, 37(21):7002-7013 (2009).
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Steinbiss S, Kastens S, and Kurtz S.
LTRsift: a graphical user interface for semi-automatic classification and postprocessing of de novo detected LTR retrotransposons.
Mob DNA. 2012 Nov 7;3(1):18.
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