Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DgriCAF1    Primary Table: refseqRNA    Row Count: 22,952   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom scaffold_10030varchar(255) values
chromStart 5474int(10) unsigned range
chromEnd 22595int(10) unsigned range
name XM_043215799varchar(255) values
score 996int(10) unsigned range
strand +char(1) values
thickStart 5624int(10) unsigned range
thickEnd 22528int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 17int(10) unsigned range
blockSizes 224,373,179,247,99,288,102,...longblob  
chromStarts 0,1276,1734,2905,3222,3377,...longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585scaffold_10030547422595XM_043215799996+562422528017224,373,179,247,99,288,102,209,164,127,206,218,225,225,158,118,163,0,1276,1734,2905,3222,3377,3736,9665,9931,10280,11095,11359,11633,11920,16281,16781,16958,
585scaffold_10030593022595XM_043215800996+59702252801796,373,179,247,99,288,102,209,164,127,206,218,225,225,158,118,163,0,820,1278,2449,2766,2921,3280,9209,9475,9824,10639,10903,11177,11464,15825,16325,16502,
585scaffold_100302552426756XM_0432157921000-2675526756011232,0,
585scaffold_1003211091XM_043215420984-110910421,20,38,1005,0,22,47,85,
585scaffold_10066177936XM_0019979181000+31667903130,127,434,0,138,325,
585scaffold_10069539930XR_0043654621000+5395390326,129,61,0,67,330,
585scaffold_100759901480XM_0432162921000-1030144401490,0,
585scaffold_1008603207XM_043214387996-03207021056,751,0,2456,
585scaffold_100907586XM_001998033998-758601579,0,
585scaffold_1009623055655XR_006246861978-2305230503127,109,188,0,2989,3162,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. grimshawi transcripts from the NCBI RefSeq database were aligned against the D. grimshawi assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. grimshawi transcripts were mapped against the D. grimshawi genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_018153295.1.