Schema for genBlastG Genes - genBlastG Gene Predictions
  Database: DkikGB2    Primary Table: genblastg    Row Count: 30,173   Data last updated: 2024-01-05
fieldexampleSQL type info
bin 585smallint(5) unsigned range
name gbg_CG4783-PB_0varchar(255) values
chrom AFFH02000335varchar(255) values
strand -char(1) values
txStart 35160int(10) unsigned range
txEnd 35457int(10) unsigned range
cdsStart 35160int(10) unsigned range
cdsEnd 35457int(10) unsigned range
exonCount 2int(10) unsigned range
exonStarts 35160,35398,longblob  
exonEnds 35341,35457,longblob  

Sample Rows
 
binnamechromstrandtxStarttxEndcdsStartcdsEndexonCountexonStartsexonEnds
585gbg_CG4783-PB_0AFFH02000335-35160354573516035457235160,35398,35341,35457,
585gbg_CG4783-PA_1AFFH02000335-35160354573516035457235160,35398,35341,35457,
585gbg_CG46042-PC_2AFFH02000335+41773421354177342135241773,41894,41825,42135,
585gbg_Hs6st-PA_3AFFH02000335+48691582514869158251548691,48841,49060,49668,58115,48808,48997,49609,49853,58251,
585gbg_Hs6st-PB_4AFFH02000335+48691582514869158251548691,48841,49060,49668,58115,48808,48997,49609,49853,58251,
585gbg_mira-PA_5AFFH02000335+53149556545314955654153149,55654,
585gbg_mira-PC_6AFFH02000335+53149556545314955654253149,55006,54934,55654,
585gbg_mira-PB_7AFFH02000335+53149556545314955654253149,55024,54934,55654,
585gbg_CG4459-PA_8AFFH02000335+59961621795996162179559961,60094,60700,60943,61628,59963,60640,60855,61570,62179,
585gbg_CG4462-PA_9AFFH02000335+64891678876489167887464891,65637,67284,67489,65570,66144,67428,67887,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

genBlastG Genes (genblastg) Track Description
 

Description

D. melanogaster proteins from FlyBase were aligned against each scaffold in the D. kikkawai (DkikGB2) assembly and the predicted gene models were constructed using genBlastG.

Methods

D. melanogaster protein sequences were aligned against the D. kikkawai (DkikGB2) genome assembly using WU-TBLASTN with the following parameters:

-B=1000 -V=1000 -hspmax=5000
-hspsepSmax=50000 -hspsepQmax=1000
-altscore='any * -999' -nogaps -hitdist=40
-matrix=BLOSUM80 -Q=12 -R=2 -e 1e-10
-wordmask=seg+xnu -topComboN=1 -links
-notes -warnings -novalidctxok

The WU-TBLASTN results were then analyzed by genBlastG using the following parameters: -P wublast -r 1 -i 30 -pro -gff -e 1e-10

References

She R, Chu JS, Uyar B, Wang J, Wang K, Chen N. genBlastG: using BLAST searches to build homologous gene models. Bioinformatics. 2011 Aug 1;27(15):2141-3.