Schema for LTRharvest - LTR Fragments Identified by LTRharvest
  Database: DkikGB2    Primary Table: ltrdigest_TSD    Row Count: 713   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom AFFH02000165varchar(255) values
chromStart 6379int(10) unsigned range
chromEnd 13095int(10) unsigned range
name repeat_region1_TSDvarchar(255) values
score 1000int(10) unsigned range
strand -char(1) values
thickStart 6379int(10) unsigned range
thickEnd 13095int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 2int(10) unsigned range
blockSizes 4,4longblob  
chromStarts 0,6712longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585AFFH02000165637913095repeat_region1_TSD1000-637913095024,40,6712
585AFFH020001651660819789repeat_region2_TSD1000+1660819789024,40,3177
585AFFH020001652278533375repeat_region3_TSD1000+2278533375024,40,10586
585AFFH02000402923078repeat_region4_TSD1000-923078024,40,2982
585AFFH0200040540205431repeat_region5_TSD1000-40205431024,40,1407
585AFFH020005541242216230repeat_region6_TSD1000-1242216230024,40,3804
585AFFH02000593596911916repeat_region7_TSD1000+596911916025,50,5942
585AFFH0200060213708762repeat_region8_TSD1000+13708762024,40,7388
585AFFH02000679210014017repeat_region9_TSD1000-210014017024,40,11913
585AFFH0200083220737494repeat_region10_TSD1000+20737494024,40,5417

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description
 

Description

LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. kikkawai genome using the following parameters:

-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best

Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters:

-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50

The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters:

-psmall 80 -plarge 30

References