Schema for genBlastG Genes - genBlastG Gene Predictions
  Database: DmojCAF1    Primary Table: genblastg    Row Count: 29,680   Data last updated: 2024-01-05
fieldexampleSQL type info
bin 585smallint(5) unsigned range
name gbg_Cyp6a21-PA_0varchar(255) values
chrom scaffold_1740varchar(255) values
strand -char(1) values
txStart 1857int(10) unsigned range
txEnd 3428int(10) unsigned range
cdsStart 1857int(10) unsigned range
cdsEnd 3428int(10) unsigned range
exonCount 2int(10) unsigned range
exonStarts 1857,2332,longblob  
exonEnds 2276,3428,longblob  

Sample Rows
 
binnamechromstrandtxStarttxEndcdsStartcdsEndexonCountexonStartsexonEnds
585gbg_Cyp6a21-PA_0scaffold_1740-185734281857342821857,2332,2276,3428,
585gbg_Cyp6a9-PB_1scaffold_1740-185734281857342821857,2332,2276,3428,
585gbg_CG10466-PA_2scaffold_1779+731207173120712731,2040,1153,2071,
585gbg_CG10466-PB_3scaffold_1779+731207173120712731,2040,1153,2071,
585gbg_CG11700-PB_4scaffold_1786+937203293720321937,2032,
585gbg_Ccz1-PB_5scaffold_1786+241439602414396052414,2558,2721,2981,3505,2498,2656,2948,3446,3960,
585gbg_Lamtor4-PB_6scaffold_1786-393145113931451143931,4040,4097,4365,4010,4061,4304,4511,
585gbg_CDase-PC_7scaffold_1801-400275940027595400,634,1310,2240,2633,584,1248,2177,2564,2759,
585gbg_CDase-PF_8scaffold_1801-400275940027595400,634,1310,2240,2633,584,1248,2177,2564,2759,
585gbg_CDase-PE_9scaffold_1801-400275940027595400,634,1310,2240,2633,584,1248,2177,2564,2759,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

genBlastG Genes (genblastg) Track Description
 

Description

D. melanogaster proteins from FlyBase were aligned against each scaffold in the D. mojavensis (DmojCAF1) assembly and the predicted gene models were constructed using genBlastG.

Methods

D. melanogaster protein sequences were aligned against the D. mojavensis (DmojCAF1) genome assembly using WU-TBLASTN with the following parameters:

-B=1000 -V=1000 -hspmax=5000
-hspsepSmax=50000 -hspsepQmax=1000
-altscore='any * -999' -nogaps -hitdist=40
-matrix=BLOSUM80 -Q=12 -R=2 -e 1e-10
-wordmask=seg+xnu -topComboN=1 -links
-notes -warnings -novalidctxok

The WU-TBLASTN results were then analyzed by genBlastG using the following parameters: -P wublast -r 1 -i 30 -pro -gff -e 1e-10

References

She R, Chu JS, Uyar B, Wang J, Wang K, Chen N. genBlastG: using BLAST searches to build homologous gene models. Bioinformatics. 2011 Aug 1;27(15):2141-3.