Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DobsGB1    Primary Table: DmelTranscriptsBLAT    Row Count: 31,590   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom BDQP01000001varchar(255) values
chromStart 11216int(10) unsigned range
chromEnd 32835int(10) unsigned range
name Ser-RBvarchar(255) values
score 714int(10) unsigned range
strand +char(1) values
thickStart 14011int(10) unsigned range
thickEnd 32224int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 30int(11) range
blockSizes 17,53,49,66,83,117,190,55,3...longblob  
chromStarts 0,17,83,2765,3024,6988,7752...longblob  
id FBtr0344121varchar(255) values
description FBgn0004197longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
BDQP010000011121632835Ser-RB714+140113222403017,53,49,66,83,117,190,55,316,134,182,61,3,129,56,36,482,32,103,98,369,519,57,755,14,33,21,13,45,570,17,83,2765,3024,6988,7752,12075,12455,14697,14969,15445,15951,16699,16872,16931,16985,17627,17758,17933,19101,19559,20114,2023 ...FBtr0344121FBgn0004197
BDQP010000011130132835Ser-RA714+140113222402845,66,83,117,190,55,316,134,182,61,3,129,56,36,482,32,103,98,369,519,57,755,14,33,21,13,45,570,2680,2939,6903,7667,11990,12370,14612,14884,15360,15866,16614,16787,16846,16900,17542,17673,17848,19016,19474,20029,20147,2092 ...FBtr0085128FBgn0004197
BDQP010000013791938885spn-D-RA398-379193888505173,124,152,108,1140,276,463,673,852FBtr0085116FBgn0003482
BDQP010000013933639670CG34293-RA518+39338396610299,420,292FBtr0112489FBgn0085322
BDQP010000013933639670CG34293-RB518+39338396610299,420,292FBtr0339263FBgn0085322
BDQP010000013977941247ppk31-RB568-397794124706144,105,4,654,207,1110,206,365,373,1094,1357FBtr0113383FBgn0051065
BDQP010000014279843713CG5909-RA458-42804437130669,222,86,180,78,1350,75,331,429,690,780FBtr0085114FBgn0039495
BDQP010000014600347348grass-RA592-460034730405425,186,50,214,1710,499,768,883,1174FBtr0085111FBgn0039494
BDQP010000014600347348grass-RB592-460034734805425,186,50,214,1710,499,768,883,1174FBtr0085112FBgn0039494
BDQP010000014814751877Men-b-RA708-49657518770939,36,31,132,297,151,1052,90,570,154,1509,1617,1821,2188,2417,3532,3673FBtr0085110FBgn0029155

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. obscura genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. obscura genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.