Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DperCAF1    Primary Table: refseqRNA    Row Count: 24,235   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom super_0varchar(255) values
chromStart 31789int(10) unsigned range
chromEnd 32592int(10) unsigned range
name XM_002012702varchar(255) values
score 1000int(10) unsigned range
strand +char(1) values
thickStart 31811int(10) unsigned range
thickEnd 32191int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 2int(10) unsigned range
blockSizes 217,527,longblob  
chromStarts 0,276,longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585super_03178932592XM_0020127021000+318113219102217,527,0,276,
585super_03438842941XM_0269841131000-347744284106408,351,2535,196,1536,782,0,465,913,3843,4097,7771,
585super_03438863039XM_0269841161000-347743999806408,351,2535,196,1536,53,0,465,913,3843,4097,28598,
585super_03438842936XM_0269841141000-347744284107408,351,2535,196,571,839,777,0,465,913,3843,4097,4794,7771,
585super_03438842937XM_0269841151000-347744284106408,222,2502,196,1536,778,0,465,913,3843,4097,7771,
585super_03438842936XM_0020127031000-347744284106408,351,2502,196,1536,777,0,465,913,3843,4097,7771,
585super_05367263041XM_026984117998-538195511904949,4,713,55,0,949,953,9314,
585super_06022462371XM_026984119978+616566214905107,307,974,80,511,0,169,476,1502,1636,
585super_06022462903XR_003408372990+602246022406107,307,974,80,409,383,0,169,476,1502,1636,2296,
585super_06359665816XM_0020127061000+636406571402322,1897,0,323,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. persimilis transcripts from the NCBI RefSeq database were aligned against the D. persimilis assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. persimilis transcripts were mapped against the D. persimilis genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_003286085.1.