Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DserGB1    Primary Table: DmelTranscriptsBLAT    Row Count: 33,166   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom MTTC01000009varchar(255) values
chromStart 28638int(10) unsigned range
chromEnd 92302int(10) unsigned range
name Wnt5-RBvarchar(255) values
score 488int(10) unsigned range
strand -char(1) values
thickStart 28638int(10) unsigned range
thickEnd 92302int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 8int(11) range
blockSizes 57,105,57,213,63,123,201,174longblob  
chromStarts 0,62154,62496,62571,63016,6...longblob  
id FBtr0343046varchar(255) values
description FBgn0010194longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
MTTC010000092863892302Wnt5-RB488-28638923020857,105,57,213,63,123,201,1740,62154,62496,62571,63016,63109,63289,63490FBtr0343046FBgn0010194
MTTC010000092863892302Wnt5-RA488-28638923020857,105,57,213,63,123,201,1740,62154,62496,62571,63016,63109,63289,63490FBtr0074623FBgn0010194
MTTC01000009164697177206CG10417-RA598+16469717720606111,273,81,114,480,1350,7097,7379,7514,8196,12374FBtr0086091FBgn0033021
MTTC01000009164697177206CG10417-RB598+16469717720606111,273,81,114,480,1350,7097,7379,7514,8196,12374FBtr0086092FBgn0033021
MTTC01000009263516263849CG10395-RD486-26351626384902174,1470,186FBtr0347209FBgn0033019
MTTC01000009263516263849CG10395-RE486-26351626384902174,1470,186FBtr0347210FBgn0033019
MTTC01000009943659944294Tsp42Ef-RA570-94365994429402123,5120,123FBtr0089023FBgn0033127
MTTC01000009973719974121GlcAT-S-RA392-97371997412104132,11,99,1290,133,174,273FBtr0079841FBgn0032135
MTTC01000009973719974195GlcAT-S-RB384-97371997417505132,11,99,129,210,133,174,273,455FBtr0079842FBgn0032135
MTTC01000009973719974195GlcAT-S-RC378-97371997417505132,11,99,129,210,133,174,273,455FBtr0302199FBgn0032135

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. serrata genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. serrata genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.