Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DsuzGB1    Primary Table: refseqRNA    Row Count: 30,350   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom AWUT01012676varchar(255) values
chromStart 14309int(10) unsigned range
chromEnd 44845int(10) unsigned range
name XM_036822623varchar(255) values
score 1000int(10) unsigned range
strand -char(1) values
thickStart 14324int(10) unsigned range
thickEnd 44753int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 4int(10) unsigned range
blockSizes 88,334,142,206,longblob  
chromStarts 0,7454,7841,30330,longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585AWUT010126761430944845XM_0368226231000-14324447530488,334,142,206,0,7454,7841,30330,
585AWUT010126762156344845XM_0170821201000-216564475304115,334,142,206,0,200,587,23076,
585AWUT010126762176344843XM_0368226181000-217634475303325,142,204,0,387,22876,
585AWUT010126762176344845XM_0368226221000-217634475303334,142,206,0,387,22876,
585AWUT010126762176344843XM_0368226171000-217634475303334,142,204,0,387,22876,
585AWUT01013449810066641XM_0170841801000+82786662607265,611,634,193,209,346,83,0,340,48565,49255,49506,49766,58458,
585AWUT01013449810260179XM_036821409998+82786007208263,611,634,193,209,810,158,114,0,338,48563,49253,49504,49764,50629,51963,
585AWUT01013449810460673XM_036821410990+827860564010261,611,634,193,209,158,145,126,189,181,0,336,48561,49251,49502,50627,51764,51961,52145,52388,
585AWUT01013449810560673XM_036821411986+82786056406260,611,145,126,189,181,0,335,51763,51960,52144,52387,
585AWUT01013449810560673XM_017084179992+827860564011260,611,634,193,209,810,158,145,126,189,181,0,335,48560,49250,49501,49761,50626,51763,51960,52144,52387,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. suzukii transcripts from the NCBI RefSeq database were aligned against the D. suzukii assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. suzukii transcripts were mapped against the D. suzukii genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_013340165.1.