Schema for LTRharvest - LTR Fragments Identified by LTRharvest
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Database: DsuzGB1 Primary Table: ltrdigest_PBS Row Count: 81   Data last updated: 2022-10-20
field | example | SQL type | info |
bin | 585 | smallint(5) unsigned | range |
chrom | AWUT01015930 | varchar(255) | values |
chromStart | 13816 | int(10) unsigned | range |
chromEnd | 13827 | int(10) unsigned | range |
name | LTR_retrotransposon29_PBS | varchar(255) | values |
score | 1000 | int(10) unsigned | range |
strand | - | char(1) | values |
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Sample Rows
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bin | chrom | chromStart | chromEnd | name | score | strand |
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585 | AWUT01015930 | 13816 | 13827 | LTR_retrotransposon29_PBS | 1000 | - |
585 | AWUT01016771 | 2515 | 2530 | LTR_retrotransposon37_PBS | 1000 | + |
585 | AWUT01018330 | 1817 | 1828 | LTR_retrotransposon51_PBS | 1000 | - |
585 | KI419162 | 3937 | 3948 | LTR_retrotransposon58_PBS | 1000 | - |
585 | KI419198 | 24873 | 24884 | LTR_retrotransposon67_PBS | 1000 | - |
585 | KI419275 | 75515 | 75530 | LTR_retrotransposon133_PBS | 1000 | + |
585 | KI419312 | 104607 | 104618 | LTR_retrotransposon159_PBS | 1000 | - |
586 | KI419331 | 191602 | 191613 | LTR_retrotransposon175_PBS | 1000 | - |
586 | KI419349 | 226429 | 226447 | LTR_retrotransposon187_PBS | 1000 | + |
591 | KI419354 | 902481 | 902492 | LTR_retrotransposon204_PBS | 1000 | - |
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Note: all start coordinates in our database are 0-based, not
1-based. See explanation
here.
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LTRharvest (ltrdigest) Track Description
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Description
LTRharvest
is used to identify putative long terminal repeats (LTR)
retrotransposons in the D. suzukii genome using the following parameters:
-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7
-mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best
Additional features within the LTR retrotransposons are annotated by
LTRdigest
with the following parameters:
-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30
-pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5
-pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6
-maxgaplen 50
The subset of candidates that show significant similarity to Pfam protein domains
within LTR retrotransposons are selected and then clustered using the ltrclustering program
with the following parameters:
-psmall 80 -plarge 30
References
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Ellinghaus D, Kurtz S, and Willhoeft U.
LTRharvest,
an efficient and flexible software for de novo detection of LTR retrotransposons.
BMC Bioinformatics, 9:18, 2008.
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Steinbiss S, Willhoeft U, Gremme G, and Kurtz S.
Fine-grained
annotation and classification of de novo predicted LTR retrotransposons.
Nucleic Acids Research, 37(21):7002-7013 (2009).
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Steinbiss S, Kastens S, and Kurtz S.
LTRsift: a graphical user interface for semi-automatic classification and postprocessing of de novo detected LTR retrotransposons.
Mob DNA. 2012 Nov 7;3(1):18.
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