Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DtakGB2    Primary Table: refseqRNA    Row Count: 14,977   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom AFFI02000001varchar(255) values
chromStart 5617int(10) unsigned range
chromEnd 7196int(10) unsigned range
name XR_001769870varchar(255) values
score 996int(10) unsigned range
strand +char(1) values
thickStart 5617int(10) unsigned range
thickEnd 5617int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 5int(10) unsigned range
blockSizes 77,19,50,69,124,longblob  
chromStarts 0,1181,1263,1386,1455,longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585AFFI0200000156177196XR_001769870996+561756170577,19,50,69,124,0,1181,1263,1386,1455,
585AFFI020000091976520199XM_017144793968+198332019502218,139,0,295,
585AFFI020000092124925710XM_017143581968+21249254810672,135,360,194,112,102,0,2820,3035,4046,4247,4359,
585AFFI0200002118501957XR_0064124521000+1850185001107,0,
585AFFI0200002118501957XR_0064125751000+1850185001107,0,
585AFFI0200002118501957XR_0064125741000+1850185001107,0,
585AFFI0200002118501957XR_0064125731000+1850185001107,0,
585AFFI0200002152158367XM_017147192992-52158363051103,153,420,339,215,0,1165,1732,2241,2937,
585AFFI020000211001417051XM_017148396988+102181639904177,914,594,532,0,178,5911,6505,
585AFFI020000341389216718XM_044392841976+1389216718022618,207,0,2619,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. takahashii transcripts from the NCBI RefSeq database were aligned against the D. takahashii assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. takahashii transcripts were mapped against the D. takahashii genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_018152695.1.