Schema for LTRharvest - LTR Fragments Identified by LTRharvest
  Database: DvirCAF1    Primary Table: ltrdigest_TSD    Row Count: 1,250   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom scaffold_10054varchar(255) values
chromStart 1204int(10) unsigned range
chromEnd 5906int(10) unsigned range
name repeat_region1_TSDvarchar(255) values
score 1000int(10) unsigned range
strand +char(1) values
thickStart 1204int(10) unsigned range
thickEnd 5906int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 2int(10) unsigned range
blockSizes 4,4longblob  
chromStarts 0,4698longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585scaffold_1005412045906repeat_region1_TSD1000+12045906024,40,4698
585scaffold_1011111217476repeat_region2_TSD1000-11217476024,40,6351
585scaffold_101273185788repeat_region3_TSD1000+3185788024,40,5466
585scaffold_101565236936repeat_region4_TSD1000-5236936024,40,6409
585scaffold_1024009570repeat_region5_TSD1000+09570024,40,9566
585scaffold_10257706915149repeat_region6_TSD1000+706915149024,40,8076
585scaffold_10304657428repeat_region7_TSD1000+657428024,40,7359
585scaffold_103089997806repeat_region8_TSD1000+9997806024,40,6803
585scaffold_1030913508510repeat_region9_TSD1000-13508510024,40,7156
585scaffold_103222733233723repeat_region10_TSD1000+2733233723024,40,6387

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

LTRharvest (ltrdigest) Track Description
 

Description

LTRharvest is used to identify putative long terminal repeats (LTR) retrotransposons in the D. virilis genome using the following parameters:

-seed 76 -minlenltr 116 -maxlenltr 800 -mindistltr 2280 -maxdistltr 8773 -similar 91 -xdrop 7 -mat 2 -mis -2 -ins -3 -del -3 -mintsd 4 -maxtsd 20 -vic 60 -overlaps best

Additional features within the LTR retrotransposons are annotated by LTRdigest with the following parameters:

-pptradius 30 -pptlen 8 30 -pptrprob 0.97 -uboxlen 3 30 -pptuprob 0.91 -pbsradius 30 -pbsalilen 11 30 -pbsoffset 0 5 -pbstrnaoffset 0 40 -pbsmaxedist 1 -pbsmatchscore 5 -pbsmismatchscore -10 -pbsinsertionscore -20 -pbsdeletionscore -20 -pdomevalcutoff 1e-6 -maxgaplen 50

The subset of candidates that show significant similarity to Pfam protein domains within LTR retrotransposons are selected and then clustered using the ltrclustering program with the following parameters:

-psmall 80 -plarge 30

References