Schema for D. mel Transcripts - BLAT Alignment of D. melanogaster Transcripts
  Database: DyakCAF1    Primary Table: DmelTranscriptsBLAT    Row Count: 32,442   Data last updated: 2024-01-05
fieldexampleSQL type info
chrom chr2Lvarchar(255) values
chromStart 8435int(10) unsigned range
chromEnd 16146int(10) unsigned range
name l(2)gl-RBvarchar(255) values
score 844int(10) unsigned range
strand -char(1) values
thickStart 8654int(10) unsigned range
thickEnd 14685int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 12int(11) range
blockSizes 42,119,109,443,641,4,104,11...longblob  
chromStarts 0,219,401,844,1349,2013,226...longblob  
id FBtr0078166varchar(255) values
description FBgn0002121longblob  

Sample Rows
 
chromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStartsiddescription
chr2L843516146l(2)gl-RB844-86541468501242,119,109,443,641,4,104,1194,776,93,20,300,219,401,844,1349,2013,2260,2421,3673,6195,7660,7681FBtr0078166FBgn0002121
chr2L843515922l(2)gl-RF842-86541282301142,119,109,443,641,4,104,1194,776,93,420,219,401,844,1349,2013,2260,2421,3673,6195,7445FBtr0078169FBgn0002121
chr2L843516146l(2)gl-RD842-86541282301342,119,109,443,641,4,104,1194,776,93,42,20,300,219,401,844,1349,2013,2260,2421,3673,6195,7445,7660,7681FBtr0078167FBgn0002121
chr2L843516146l(2)gl-RE842-86541282301342,119,109,443,641,4,104,1194,776,93,42,20,300,219,401,844,1349,2013,2260,2421,3673,6195,7445,7660,7681FBtr0078168FBgn0002121
chr2L843516146l(2)gl-RC842-86541468501442,119,109,443,641,4,104,1194,776,113,11,42,20,300,219,401,844,1349,2013,2260,2421,3673,6164,6308,7445,7660,7681FBtr0078170FBgn0002121
chr2L843516146l(2)gl-RA842-86541468501542,119,109,443,641,4,104,1194,776,113,42,67,42,20,300,219,401,844,1349,2013,2260,2421,3673,6164,7173,7270,7445,7660,7681FBtr0078171FBgn0002121
chr2L843516146l(2)gl-RG846-86541468501242,119,109,443,641,4,104,1194,776,113,20,300,219,401,844,1349,2013,2260,2421,3673,6164,7660,7681FBtr0306589FBgn0002121
chr2L843516146l(2)gl-RH838-86541468501342,119,109,443,641,4,104,1194,776,113,11,104,300,219,401,844,1349,2013,2260,2421,3673,6164,6308,7576,7681FBtr0306590FBgn0002121
chr2L843516146l(2)gl-RI842-86541468501342,119,109,443,641,4,104,1194,776,113,42,20,300,219,401,844,1349,2013,2260,2421,3673,6164,7445,7660,7681FBtr0306591FBgn0002121
chr2L843516146l(2)gl-RJ840-86541468501642,119,109,443,641,4,104,1194,776,113,11,42,67,42,20,300,219,401,844,1349,2013,2260,2421,3673,6164,6277,7173,7270,7445,7660,7681FBtr0306592FBgn0002121

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

D. mel Transcripts (DmelTranscriptsBLAT) Track Description
 

Description

D. melanogaster transcripts annotated by FlyBase were aligned against the D. yakuba genome assembly using a translated BLAT search. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. melanogaster transcripts were mapped against the D. yakuba genome assembly using BLAT with the following parameters: -q=rnax -t=dnax -mask=lower

The transcript alignments are analyzed by pslReps to identify the best alignments within the genome assembly that have a minimum alignment ratio of 0.25 (-minAli=0.25). The alignments are then filtered by pslCDnaFilter using the following parameters:

  -minId=0.35 -minCover=0.15 -localNearBest=0.010 -minQSize=20
  -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT--the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.