Schema for RefSeq Genes - BLAT Alignments of NCBI RefSeq Genes
  Database: DyakCAF1    Primary Table: refseqRNA    Row Count: 30,001   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 750int(10) unsigned range
chromEnd 3293int(10) unsigned range
name XR_006245117varchar(255) values
score 982int(10) unsigned range
strand -char(1) values
thickStart 750int(10) unsigned range
thickEnd 750int(10) unsigned range
reserved 0int(10) unsigned range
blockCount 5int(10) unsigned range
blockSizes 1626,40,108,313,317,longblob  
chromStarts 0,1626,1680,1788,2226,longblob  

Sample Rows
 
binchromchromStartchromEndnamescorestrandthickStartthickEndreservedblockCountblockSizeschromStarts
585chr2L7503293XR_006245117982-750750051626,40,108,313,317,0,1626,1680,1788,2226,
585chr2L7503292XR_005560275984-75075006855,681,40,108,313,316,0,945,1626,1680,1788,2226,
585chr2L7503293XR_005560274984-75075006886,681,40,108,313,317,0,945,1626,1680,1788,2226,
585chr2L681616159XM_0020873291000-864314684091957,109,443,643,106,1192,779,161,295,0,2020,2463,2968,3877,4040,5290,7784,9048,
585chr2L893916160XM_0151978721000-90451468408129,443,643,106,1192,779,161,296,0,340,845,1754,1917,3167,5661,6925,
585chr2L2084548858XM_0393712651000-2096247705013285,199,438,226,194,87,444,47,484,197,557,102,95,0,349,645,1658,2393,2642,6526,7252,7366,11195,11657,26790,27918,
585chr2L2084554906XM_0393712531000-2096247705014285,199,438,229,194,87,801,444,47,484,197,557,102,238,0,349,645,2060,2393,2642,4098,6526,7252,7366,11195,11657,26790,33823,
585chr2L2084561792XM_0393712541000-2096247705014285,199,438,229,194,87,801,444,47,484,197,557,102,130,0,349,645,2060,2393,2642,4098,6526,7252,7366,11195,11657,26790,40817,
585chr2L2084548740XM_0393712551000-2096247705014285,199,438,229,194,87,801,444,47,484,197,557,102,219,0,349,645,2060,2393,2642,4098,6526,7252,7366,11195,11657,26790,27676,
585chr2L2084548860XM_0393712561000-2096247705014285,199,438,226,194,87,801,444,47,484,197,557,102,97,0,349,645,1658,2393,2642,4098,6526,7252,7366,11195,11657,26790,27918,

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

RefSeq Genes (refseqRNA) Track Description
 

Description

D. yakuba transcripts from the NCBI RefSeq database were aligned against the D. yakuba assembly using BLAT. The thicker boxes denote the coding regions within the transcripts and the thinner boxes denote the untranslated regions.

Methods

D. yakuba transcripts were mapped against the D. yakuba genome assembly using BLAT with the following parameters:

    -q=rna -fine -minScore=20 -stepSize=5

The transcript alignments are analyzed by pslReps using the following parameter:

    -minCover=0.15 -minAli=0.98 -nearTop=0.001

The alignments are then filtered by pslCDnaFilter using the following parameters:

    -minId=0.95 -minCover=0.15 -localNearBest=0.001 \
    -minQSize=20 -ignoreIntrons -repsAsMatch -ignoreNs -bestOverlap

References

Kent WJ. BLAT — the BLAST-like alignment tool. Genome Res. 2002 Apr;12(4):656-64.

The transcripts were obtained from the NCBI FTP site under the RefSeq assembly accession number GCF_016746365.2.