Schema for R-Loop DRIP-Seq (S2) - R-Loop DRIP-Seq in S2 Cells
  Database: dm6    Primary Table: CSE99014_dH1_RNH1_untreated_macs    Row Count: 43,470   Data last updated: 2022-10-20
fieldexampleSQL type info
bin 585smallint(5) unsigned range
chrom chr2Lvarchar(255) values
chromStart 5101int(10) unsigned range
chromEnd 5347int(10) unsigned range
name CSE99014_dH1_RNH1_untreated...varchar(255) values
score 36int(10) unsigned range
strand .char(1) values
signalValue 2.17467float range
pValue 5.08059float range
qValue 3.67268float range
peak 219int(11) range

Sample Rows
 
binchromchromStartchromEndnamescorestrandsignalValuepValueqValuepeak
585chr2L51015347CSE99014_dH1_RNH1_untreated_macs_peak_1b36.2.174675.080593.67268219
585chr2L51015347CSE99014_dH1_RNH1_untreated_macs_peak_1a89.3.1433110.91258.9153372
585chr2L54975632CSE99014_dH1_RNH1_untreated_macs_peak_273.2.818599.182757.3363267
585chr2L57735994CSE99014_dH1_RNH1_untreated_macs_peak_3212.4.2994624.021421.2029101
585chr2L989410052CSE99014_dH1_RNH1_untreated_macs_peak_4118.3.683814.091611.852469
585chr2L1687017121CSE99014_dH1_RNH1_untreated_macs_peak_582.3.1639110.18258.2455589
585chr2L1972819889CSE99014_dH1_RNH1_untreated_macs_peak_6119.3.7175914.244911.98977
585chr2L3622736392CSE99014_dH1_RNH1_untreated_macs_peak_751.2.493846.736625.1351166
585chr2L4734247484CSE99014_dH1_RNH1_untreated_macs_peak_888.2.6981110.85128.8590661
585chr2L102536102748CSE99014_dH1_RNH1_untreated_macs_peak_9101.3.2118912.285210.1802110

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

R-Loop DRIP-Seq (S2) (rloopDRIPSeq) Track Description
 

Description

These tracks show the putative locations of R-loops based on DRIP-Seq data from Drosophila melanogaster S2 Cells. The samples that were treated with RNase H served as additional controls for identifying R-loops. The regions that are enriched in the DRIP-Seq reads were identified by MACS2 and Peakzilla. The log likelihood ratio evidence tracks were produced by MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.

The DRIP-Seq datasets were obtained from the Gene Expression Omnibus database at NCBI under the accession number GSE99014.

References

Bayona-Feliu A, Casas-Lamesa A, Reina O, Bernués J, Azorin F. Linker histone H1 prevents R-loop accumulation and genome instability in heterochromatin. Nat Commun. 2017 Aug 18;8(1):283.

Bardet AF, Steinmann J, Bafna S, Knoblich JA, Zeitlinger J, Stark A. Identification of transcription factor binding sites from ChIP-seq data at high resolution. Bioinformatics. 2013 Nov 1;29(21):2705-13.

Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS). Genome Biol. 2008;9(9):R137.