MUSIC Histone Modifications Peaks Track Settings
 
ChIP-Seq Peaks Identified by MUSIC   (All ChIP Seq Tracks)

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 H3K4me2 Peaks  Punctated H3K4me2 Peaks Identified by MUSIC   Schema 
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 H3K9me2 Peaks  Broad H3K9me2 Peaks Identified by MUSIC   Schema 
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 H3K27me3 Peaks  Broad H3K27me3 Peaks Identified by MUSIC   Schema 

Description

ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using MUSIC in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications.

Reads from the two replicates were merged into a single file prior to the analysis with MUSIC. Only the subset of scaffolds larger than 100kb in the D. melanogaster assembly were analyzed by MUSIC. Broad peaks for H3K9me2 and H3K27me3 were identified using the -get_optimal_broad_ERs option. Punctated peaks for H3K4me2 were identified using the -get_optimal_punctate_ERs option.

References

Harmanci A, Rozowsky J, Gerstein M. MUSIC: identification of enriched regions in ChIP-Seq experiments using a mappability-corrected multiscale signal processing framework. Genome Biol. 2014;15(10):474.