Description
D. ananassae PacBio reads generated by the Genome Institute at Washington
University were aligned against the D. ananassae CAF1 genome assembly
using BLASR. For each
BLASR aligned read, we calculated the size of the unaligned region compared
to the length of the entire read.
The color of the read in this track corresponds to its unaligned length
| Color | Unaligned Length |
|---|
| ≥ 8000 |
| 5000-8000 |
| 3000-5000 |
| 2000-3000 |
| 1000-2000 |
| 500-1000 |
| 300-500 |
| 200-300 |
| 100-200 |
| ≤ 100 |
Methods
After constructing a bwt index for the D. ananassae CAF1 assembly,
D. ananassae PacBio reads from 8 SMRT cells were aligned against the
D. ananassae CAF1 assembly using BLASR with the following parameters:
- minPctIdentity 70
- bestn 1
References
Chaisson MJ, Tesler G.
Mapping single molecule sequencing reads using basic local alignment with
successive refinement (BLASR): application and theory.
BMC Bioinformatics. 2012 Sep 19;13:238
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