Description
D. ananassae PacBio reads generated by the Genome Institute at Washington
University were aligned against the D. ananassae CAF1 genome assembly
using BLASR.
Methods
After constructing a bwt index for the D. ananassae CAF1 assembly,
D. ananassae PacBio reads from 8 SMRT cells were aligned against the
D. ananassae CAF1 assembly using BLASR with the following parameters:
- minPctIdentity 70
- bestn 1
References
Chaisson MJ, Tesler G.
Mapping single molecule sequencing reads using basic local alignment with
successive refinement (BLASR): application and theory.
BMC Bioinformatics. 2012 Sep 19;13:238
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