RNA-Seq Coverage (Stages) Track Settings
 
RNA-Seq Coverage for Different Developmental Stages   (All RNA Seq Tracks)

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Description

These tracks show the RNA-Seq coverage for different developmental stages of D. ananassae. The RNA-Seq read coverage was normalized by a wigsum of 100,000,000 (1 million 100bp reads). The RNA-Seq reads were mapped against the D. ananassae DanaCAF1 genome assembly using HISAT2. RNA-Seq reads from multiple replicates were combined together. The read coverage tracks were produced by the bam2wig.py program in RSeQC.

The RNA-Seq data were retrieved from the NCBI Sequence Read Archive using the following accession numbers:

SRA Study AccessionDescription
SRP006203Adult Female
SRP006203Adule Male
SRP007906Wolbachia-cured Embryo

References

Brown JB. et al. Diversity and dynamics of the Drosophila transcriptome. Nature. 2014 Aug 28;512(7515):393-9.

Kumar N, Creasy T, Sun Y, Flowers M, Tallon LJ, Dunning Hotopp JC. Efficient subtraction of insect rRNA prior to transcriptome analysis of Wolbachia-Drosophila lateral gene transfer. BMC Res Notes. 2012 May 14;5:230.

Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods. 2015 Apr;12(4):357-60.

Wang L, Wang S, Li W. RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.