Melting Temp. Track Settings

Melting Temperature Estimated by dan (All Mapping and Sequencing Tracks)

Display mode:

Type of graph:
Track height:	pixels (range: 11 to 110)
Data view scaling:	Always include zero:
Vertical viewing range:	min:	max: (range: 0 to 60)
Transform function:	Transform data points by:
Windowing function:		Smoothing window:	pixels
Negate values:
Draw y indicator lines:	at y = 0.0: at y =

Graph configuration help

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Data last updated at UCSC: 2013-05-26 16:49:33

Description

This track shows the melting temperature as determined by the dan tool in the EMBOSS package using a sliding window of 9bp (windowsize=9) and a step size of 1 (shiftincrement=1) with the following parameters: dnaconc=50, saltconc=50, mintemp=55.

References

Rice, P. Longden, I. and Bleasby, A. EMBOSS: The European Molecular Biology Open Software Suite. (2000) Trends in Genetics 16, (6) pp276--277