TopHat Junctions Track Settings
 
D. suzukii TopHat Splice Junctions   (All RNA Seq Tracks)

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Show only items with score at or above:   (range: 0 to 1000)

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Description

This track was created by mapping D. suzukii RNA-Seq reads (generated by the Spotted Wing Drosophila Genome Project) against the D. suzukii DsuzGB1 assembly using Bowtie2 and TopHat. The RNA-Seq data were obtained from the NCBI Sequence Read Archive under the BioProject accession number PRJNA221549.

The TopHat junction predictions from the different libraries are filtered and merged together into a single set of predictions. The predictions are color-coded based on the number of reads supporting the junction:

ColorNumber of reads
> 1000
500-999
100-499
50-99
10-49
< 10

References

Chiu JC et al. Genome of Drosophila suzukii, the spotted wing drosophila. G3 (Bethesda). 2013 Dec 9;3(12):2257-71.

Trapnell C, Pachter L, Salzberg SL. TopHat: discovering splice junctions with RNA-Seq. Bioinformatics. 2009 May 1;25(9):1105-11.