Drosophila Conservation (28 Species) Track Settings
 
ROAST Alignments & Conservation (28 Drosophila Species)   (All Comparative Genomics tracks)

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ROAST Alignments ▾       Basewise Conservation (phyloP) ▾       Element Conservation (phastCons) ▾       Conserved Elements ▾      
 
ROAST Alignments Configuration

Species selection:  + -

  Drosophila  + -

d. arizonae
d. navojoa
d. hydei
d. virilis
d. grimshawi

  Dorsilopha  + -

d. busckii

  Sophophora  + -

d. melanogaster
d. simulans
d. sechellia
d. yakuba
d. erecta
d. eugracilis
d. biarmipes
d. suzukii
d. takahashii
d. elegans
d. rhopaloa
d. ficusphila
d. kikkawai
d. serrata
d. ananassae
d. bipectinata
d. obscura
d. pseudoobscura
d. persimilis
d. miranda
d. willistoni

Multiple alignment base-level:
Display bases identical to reference as dots
Display chains between alignments

Codon Translation:
Default species to establish reading frame:
No codon translation
Use default species reading frames for translation
Use reading frames for species if available, otherwise no translation
Use reading frames for species if available, otherwise use default species
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 4D Sites (l=10)  Conserved Elements (4D Sites, c=0.1, l=10)   Schema 
 
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 4D Sites  PhastCons Scores Based on Four-Fold Degenerate Sites   Schema 
 
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 4D Sites  PhyloP Scores Based on Four-Fold Degenerate Sites   Schema 
 
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 ROAST (28 Species)  ROAST Alignments for 28 Drosophila Species   Schema 

Description

This track shows the multiple alignments of 28 Drosophila species and measurements of evolutionary conservation using two methods (phastCons and phyloP) from the PHAST package. The pairwise sequence alignments were generated using LAST. The multiple sequence alignments were generated using the tools developed by UCSC and the Miller lab at the Penn State University Center for Comparative Genomics and Bioinformatics. Conserved elements identified by phastCons are also displayed in this track.

PhastCons is a hidden Markov model-based method that estimates the probability that each nucleotide belongs to a conserved element, based on the multiple alignment. It considers not just each individual alignment column, but also its flanking columns. By contrast, phyloP separately measures conservation at individual columns, ignoring the effects of their neighbors. As a consequence, the phyloP plots have a less smooth appearance than the phastCons plots, with more "texture"; at individual sites. The two methods have different strengths and weaknesses. PhastCons is sensitive to "runs"; of conserved sites, and is therefore effective for picking out conserved elements. PhyloP, on the other hand, is more appropriate for evaluating signatures of selection at particular nucleotides or classes of nucleotides (e.g., third codon positions, or first positions of miRNA target sites).

Another important difference is that phyloP can measure acceleration (faster evolution than expected under neutral drift) as well as conservation (slower than expected evolution). In the phyloP plots, sites predicted to be conserved are assigned positive scores (and shown in blue), while sites predicted to be fast-evolving are assigned negative scores (and shown in red). The absolute values of the scores represent -log p-values under a null hypothesis of neutral evolution. The phastCons scores, by contrast, represent probabilities of negative selection and range between 0 and 1.

Both phastCons and phyloP treat alignment gaps and unaligned nucleotides as missing data.

Missing sequence in the assemblies is highlighted in the track display by regions of yellow when zoomed out and Ns displayed at base level (see Gap Annotation, below).

Genome assemblies included in the 28-way Drosophila Conservation track

SpeciesAssembly NameGenBank AccessionUCSC Assembly
Drosophila melanogasterRelease 6 plus ISO1 MTGCA_000001215.4dm6
Drosophila simulansASM75419v2GCA_000754195.3DsimGB2
Drosophila sechelliadsec_caf1GCA_000005215.1DsecCAF1
Drosophila yakubadyak_caf1GCA_000005975.1DyakCAF1
Drosophila erectadere_caf1GCA_000005135.1DereCAF1
Drosophila eugracilisDeug_2.0GCA_000236325.2DeugGB2
Drosophila biarmipesDbia_2.0GCA_000233415.2DbiaGB2
Drosophila suzukiiDsuzukii.v01GCA_000472105.1DsuzGB1
Drosophila takahashiiDtak_2.0GCA_000224235.2DtakGB2
Drosophila elegansDele_2.0GCA_000224195.2DeleGB2
Drosophila rhopaloaDrho_2.0GCA_000236305.2DrhoGB2
Drosophila ficusphilaDfic_2.0GCA_000220665.2DficGB2
Drosophila kikkawaiDkik_2.0GCA_000224215.2DkikGB2
Drosophila serrataDser1.0GCA_002093755.1DserGB1
Drosophila ananassaedana_caf1GCA_000005115.1DanaCAF1
Drosophila bipectinataDbip_2.0GCA_000236285.2DbipGB2
Drosophila obscuraDobs_1.0GCA_002217835.1DobsGB1
Drosophila pseudoobscuraDpse_3.0GCA_000001765.2DpseGB3
Drosophila persimilisdper_caf1GCA_000005195.1DperCAF1
Drosophila mirandaDroMir_2.2GCA_000269505.2DmirGB2
Drosophila willistonidwil_caf1GCA_000005925.1DwilCAF1
Drosophila mojavensisdmoj_caf1GCA_000005175.1DmojCAF1
Drosophila arizonaeASM165402v1GCA_001654025.1DariGB1
Drosophila navojoaASM165401v1GCA_001654015.1DnavGB1
Drosophila hydeiASM278046v1GCA_002780465.1DhydGB1
Drosophila virilisdvir_caf1GCA_000005245.1DvirCAF1
Drosophila grimshawidgri_caf1GCA_000005155.1DgriCAF1
Drosophila busckiiASM127793v1GCA_001277935.1DbusGB1

Display Conventions and Configuration

In full and pack display modes, conservation scores are displayed as a wiggle track (histogram) in which the height reflects the value of the score. The conservation wiggles can be configured in a variety of ways to highlight different aspects of the displayed information. (See the "Configuring graph-based tracks" page for details.)

Pairwise alignments of each species to the D. mojavensis genome are displayed below the conservation histogram as a grayscale density plot (in pack mode) or as a wiggle (in full mode) that indicates alignment quality. In dense display mode, conservation is shown in grayscale using darker values to indicate higher levels of overall conservation as scored by phastCons.

Checkboxes on the track configuration page allow selection of the species to include in the pairwise display. The "+" and "-" buttons allow you to select or unselect multiple species at once. Note that excluding species from the pairwise display does not alter the conservation score display.

To view detailed information about the alignments at a specific position, zoom the display in to 30,000 or fewer bases, then click on the alignment.

Gap Annotation

The following display conventions are used to depict the different types of gaps in the alignment:

  • Single line: No bases in the aligned species. Possibly due to a lineage-specific insertion between the aligned blocks in the D. mojavensis genome or a lineage-specific deletion between the aligned blocks in the aligning species.
  • Double line: Aligning species has one or more unalignable bases in the gap region. Possibly due to excessive evolutionary distance between species or independent indels in the region between the aligned blocks in both species.
  • Pale yellow coloring: Aligning species has Ns in the gap region. Reflects uncertainty in the relationship between the DNA of both species, due to lack of sequence in relevant portions of the aligning species.

Genomic Breaks

Discontinuities in the genomic context (chromosome, scaffold or region) of the aligned DNA in the aligning species are shown as follows:

  • Vertical blue bar: Represents a discontinuity that persists indefinitely on either side, e.g., a large region of DNA on either side of the bar comes from a different chromosome in the aligned species due to a large-scale rearrangement.
  • Green square brackets: Enclose shorter alignments consisting of DNA from one genomic context in the aligned species nested inside a larger chain of alignments from a different genomic context. The alignment within the brackets may represent a short misalignment, a lineage-specific insertion of a transposon in the D. mojavensis genome that aligns to a paralogous copy somewhere else in the aligned species, or other similar occurrence.

Base Level

When zoomed-in to the base-level display, the track shows the base composition of each alignment. The numbers and symbols on the Gaps line indicate the lengths of gaps in the D. mojavensis sequence at those alignment positions relative to the longest non-D. mojavensis sequence. If there is sufficient space in the display, the size of the gap is shown. If the space is insufficient and the gap size is a multiple of 3, a "*" is displayed; other gap sizes are indicated by "+".

Codon translation is available in base-level display mode if the displayed region is identified as a coding segment. To display this annotation, select the species for translation from the pull-down menu in the Codon Translation configuration section at the top of the page. Then, select one of the following modes:

  • No codon translation: The gene annotation is not used; the bases are displayed without translation.
  • Use default species reading frames for translation: The annotations from the genome displayed in the "Default species to establish reading frame" pull-down menu are used to translate all the aligned species present in the alignment.
  • Use reading frames for species if available, otherwise no translation: Codon translation is performed only for those species where the region is annotated as protein coding.
  • Use reading frames for species if available, otherwise use default species: Codon translation is done on those species that are annotated as being protein coding over the aligned region using species-specific annotation; the remaining species are translated using the default species annotation.

Methods

Pairwise alignments with the D. mojavensis genome were generated for each species using LAST from repeat-masked genomic sequence. Pairwise alignments were then linked into chains using a dynamic programming algorithm that finds maximally scoring chains of gapless subsections of the alignments organized in a kd-tree.

The scoring matrix and parameters for pairwise alignment and chaining were tuned for each species based on phylogenetic distance from the reference. High-scoring chains were then placed along the genome, with gaps filled by lower-scoring chains, to produce an alignment net. For more information about the chaining and netting process and parameters for each species, see the description pages for the Chain and Net tracks.

The resulting best-in-genome pairwise alignments were progressively aligned using ROAST, following the tree topology diagrammed above, to produce multiple alignments. The multiple alignments were post-processed to add annotations indicating alignment gaps, genomic breaks, and base quality of the component sequences. An alignment summary table containing an entry for each alignment block in each species was generated to improve track display performance at large scales. Framing tables were constructed to enable visualization of codons in the multiple alignment display.

Phylogenetic Tree Model

Both phastCons and phyloP are phylogenetic methods that rely on a tree model containing the tree topology, branch lengths representing evolutionary distance at neutrally evolving sites, the background distribution of nucleotides, and a substitution rate matrix. The tree model was produced using 4-fold degenerate sites extracted from the 28-way alignments. The 4-fold degenerate sites were derived from the D. mojavensis gene annotations produced by FlyBase, filtered to select single-coverage long transcripts.

PhastCons Conservation

The phastCons program computes conservation scores based on a phylo-HMM, a type of probabilistic model that describes both the process of DNA substitution at each site in a genome and the way this process changes from one site to the next (Felsenstein and Churchill 1996, Yang 1995, Siepel and Haussler 2005). PhastCons uses a two-state phylo-HMM, with a state for conserved regions and a state for non-conserved regions. The value plotted at each site is the posterior probability that the corresponding alignment column was "generated" by the conserved state of the phylo-HMM. These scores reflect the phylogeny (including branch lengths) of the species in question, a continuous-time Markov model of the nucleotide substitution process, and a tendency for conservation levels to be autocorrelated along the genome (i.e., to be similar at adjacent sites). The general reversible (REV) substitution model was used. Unlike many conservation-scoring programs, phastCons does not rely on a sliding window of fixed size; therefore, short highly-conserved regions and long moderately conserved regions can both obtain high scores. More information about phastCons can be found in Siepel et al. 2005.

The following parameters were used in the phastCons analysis: expected-length=25, target-coverage=0.4.

PhyloP Conservation

The phyloP program supports several different methods for computing p-values of conservation or acceleration, for individual nucleotides or larger elements. Here it was used to produce separate scores at each base (--wig-scores option), considering all branches of the phylogeny rather than a particular subtree or lineage (i.e., the --subtree option was not used). The scores were computed by performing a likelihood ratio test at each alignment column (--method LRT), and scores for both conservation and acceleration were produced (--mode CONACC).

Conserved Elements

The conserved elements were predicted by running phastCons with the --viterbi option. The predicted elements are segments of the alignment that are likely to have been "generated" by the conserved state of the phylo-HMM. Each element is assigned a log-odds score equal to its log probability under the conserved model minus its log probability under the non-conserved model. The "score" field associated with this track contains transformed log-odds scores, taking values between 0 and 1000. (The scores are transformed using a monotonic function of the form a * log(x) + b.) The raw log odds scores are retained in the "name" field and can be seen on the details page or in the browser when the track's display mode is set to "pack" or "full".

Credits

This track was created using the following programs:

  • Alignment tools: LAST by Szymon M. Kiełbasa, Raymond Wan, Kengo Sato, Paul Horton and Martin C. Frith; ROAST by Minmei Hou.
  • Chaining and Netting: axtChain, chainNet by Jim Kent at UCSC.
  • Conservation scoring: phastCons, phyloP, phyloFit, tree_doctor, msa_view and other programs in PHAST by Adam Siepel.
  • MAF Annotation tools: mafAddIRows by Brian Raney, UCSC; mafAddQRows by Richard Burhans, Penn State; genePredToMafFrames by Mark Diekhans, UCSC.
  • Tree image generator: phyloPng by Galt Barber, UCSC.
  • Conservation track display: Kate Rosenbloom, Hiram Clawson (wiggle display), and Brian Raney (gap annotation and codon framing) at UCSC.

References

Phylo-HMMs, phastCons, and phyloP:

Felsenstein J, Churchill GA. A Hidden Markov Model approach to variation among sites in rate of evolution. Mol Biol Evol. 1996 Jan;13(1):93-104. PMID: 8583911

Pollard KS, Hubisz MJ, Rosenbloom KR, Siepel A. Detection of nonneutral substitution rates on mammalian phylogenies. Genome Res. 2010 Jan;20(1):110-21. PMID: 19858363; PMC: PMC2798823

Siepel A, Bejerano G, Pedersen JS, Hinrichs AS, Hou M, Rosenbloom K, Clawson H, Spieth J, Hillier LW, Richards S, et al. Evolutionarily conserved elements in vertebrate, insect, worm, and yeast genomes. Genome Res. 2005 Aug;15(8):1034-50. PMID: 16024819; PMC: PMC1182216

Siepel A, Haussler D. Phylogenetic Hidden Markov Models. In: Nielsen R, editor. Statistical Methods in Molecular Evolution. New York: Springer; 2005. pp. 325-351.

Yang Z. A space-time process model for the evolution of DNA sequences. Genetics. 1995 Feb;139(2):993-1005. PMID: 7713447; PMC: PMC1206396

Chain/Net:

Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D. Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9. PMID: 14500911; PMC: PMC208784

Multiz:

Blanchette M, Kent WJ, Riemer C, Elnitski L, Smit AF, Roskin KM, Baertsch R, Rosenbloom K, Clawson H, Green ED, et al. Aligning multiple genomic sequences with the threaded blockset aligner. Genome Res. 2004 Apr;14(4):708-15. PMID: 15060014; PMC: PMC383317

Whole genome alignment:

Kiełbasa SM1, Wan R, Sato K, Horton P, Frith MC. Adaptive seeds tame genomic sequence comparison. Genome Res. 2011 Mar;21(3):487-93. PMID: 21209072; PMC: PMC3044862