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Reference-guided and de novo transcripts were assembled by Trinity using D. biarmipes RNA-Seq reads. These assembled transcripts are mapped against the D. biarmipes DbiaGB2 assembly using BLAT.
Grabherr MG et al Full-length transcriptome assembly from RNA-Seq data without a reference genome. Nat Biotechnol. 2011 May 15;29(7):644-52.
Chen ZX et al. Comparative validation of the D. melanogaster modENCODE transcriptome annotation. Genome Res. 2014 Jul;24(7):1209-23.