Melting Temp. Track Settings
 
Melting Temperature Estimated by dan   (All Mapping and Sequencing Tracks)

Display mode:   

Type of graph:
Track height: pixels (range: 11 to 110)
Data view scaling: Always include zero: 
Vertical viewing range: min:  max:   (range: 0 to 60)
Transform function:Transform data points by: 
Windowing function: Smoothing window:  pixels
Negate values:
Draw y indicator lines:at y = 0.0:    at y =
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Data last updated at UCSC: 2014-09-21 13:37:15

Description

This track shows the melting temperature as determined by the dan tool in the EMBOSS package using a sliding window of 9bp (windowsize=9) and a step size of 1 (shiftincrement=1) with the following parameters: dnaconc=50, saltconc=50, mintemp=55.

References

Rice, P. Longden, I. and Bleasby, A. EMBOSS: The European Molecular Biology Open Software Suite. (2000) Trends in Genetics 16, (6) pp276--277