Description
This track was created by mapping D. kikkawai RNA-Seq reads (generated by
the modENCODE project) against the D. kikkawai DkikGB2 assembly using Bowtie2 and TopHat. The
RNA-Seq data were obtained from the NCBI Sequence Read Archive under the BioProject accession number
PRJNA63469.
The TopHat junction predictions from the different libraries are filtered
and merged together into a single set of predictions. The predictions are
color-coded based on the number of reads supporting the junction:
| Color | Number of reads |
|---|
| > 1000 |
| 500-999 |
| 100-499 |
| 50-99 |
| 10-49 |
| < 10 |
References
Chen ZX et al. Comparative validation
of the D. melanogaster modENCODE transcriptome annotation. Genome Res. 2014 Jul;24(7):1209-23.
Trapnell C, Pachter L, Salzberg SL.
TopHat: discovering splice junctions with RNA-Seq.
Bioinformatics. 2009 May 1;25(9):1105-11.
|