>HindIII 894 1146 5798 6532 10544 15758 -1 >EcoRI 4147 4950 5666 6119 8482 11037 -1 >EcoRV 1368 3227 7541 7602 8101 12657 -1 >SacI 2174 2231 5845 5845 8542 15592 -1