>HindIII 1063 1555 2054 3598 5379 9898 12550 -1 >EcoRI 1490 1572 3904 4852 5425 7777 12392 -1 >EcoRV 7062 7586 11219 -1 >SacI 1868 2879 3409 10942 17303 -1