>HindIII 1460 2042 2181 3020 3220 3371 3834 4914 5420 9035 12810 -1 >EcoRI 2064 2643 5191 7241 8379 12631 12790 -1 >EcoRV 2947 3066 6302 7743 9139 10565 10565 -1 >SacI 8538 9399 9680 14218 -1