>HindIII 1200 1742 2653 3751 6685 7060 10081 -1 >EcoRI 1125 1663 1705 1812 2208 2399 3748 17311 -1 >EcoRV 1199 1217 1258 1492 1730 1825 1889 2915 3283 3745 5160 6004 -1 >SacI 6870 27109 -1