PePr Histone Modifications Peaks Track Settings
 
ChIP-Seq Peaks Identified by PePr   (All ChIP Seq Tracks)

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 H3K4me2 Peaks  H3K4me2 Peaks Identified by PePr   Schema 
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 H3K9me2 Peaks  H3K9me2 Peaks Identified by PePr   Schema 
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 H3K27me3 Peaks  H3K27me3 Peaks Identified by PePr   Schema 

Description

ChIP-Seq data for H3K4me2, H3K9me2, and H3K27me3 were analyzed using PePr in order to identify regions in the D. melanogaster genome that are enriched for these histone modifications.

Reads from the two replicates for each histone modification were provided to PePr in a single run. Regions enriched in H3K4me2 were identified using PePr with the following parameters:

--peaktype=sharp --remove_artefacts

Regions enriched in H3K9me2 and H3K27me3 were identified using PePr with the following parameters:

--peaktype=broad --remove_artefacts

References

Zhang Y, Lin YH, Johnson TD, Rozek LS, Sartor MA. PePr: a peak-calling prioritization pipeline to identify consistent or differential peaks from replicated ChIP-Seq data. Bioinformatics. 2014 Sep 15;30(18):2568-75.