Melting Temp. Track Settings
 
Melting Temperature Estimated by dan   (All Mapping and Sequencing Tracks)

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Type of graph:
Track height: pixels (range: 11 to 110)
Data view scaling: Always include zero: 
Vertical viewing range: min:  max:   (range: 0 to 60)
Transform function:Transform data points by: 
Windowing function: Smoothing window:  pixels
Negate values:
Draw y indicator lines:at y = 0.0:    at y =
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Data last updated at UCSC: 2015-10-10 17:58:56

Description

This track shows the DNA melting temperature within a 9bp sliding window as estimated by the dan EMBOSS tool. The melting temperatures were determined using the following parameters:

-windowsize=9 -shiftincrement=1 -dnaconc=50 -saltconc=50 -mintemp=55

References

Rice P, Longden I, Bleasby A. EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet. 2000 Jun;16(6):276-7.