These tracks show the normalized density of SAGE reads from different developmental stages of D. melanogaster.
The datasets were produced by MachiBase and were obtained
from the NCBI BioProject database under the accession number PRJNA79391.
Each dataset was mapped to the D. melanogaster release 6 assembly using bwa and the
alignment results were analyzed by CAGEr.
Consensus promoters across all the samples were produced by the consensusClusters function in CAGEr with the following parameters:
tpmThreshold = 5, qLow = 0.1, qUp = 0.9, maxDist = 100
Ahsan B, Saito TL, Hashimoto S, Muramatsu K, Tsuda M, Sasaki A, Matsushima K, Aigaki T, Morishita S.
MachiBase: a Drosophila melanogaster 5'-end mRNA transcription database.
Nucleic Acids Res. 2009 Jan;37(Database issue):D49-53.
Haberle V, Forrest AR, Hayashizaki Y, Carninci P, Lenhard B.
CAGEr: precise TSS data retrieval and high-resolution promoterome mining for integrative analyses.
Nucleic Acids Res. 2015 Apr 30;43(8):e51.