These tracks show the read coverage from global run-on sequencing (GRO-Seq) of
D. melanogaster S2 cells. The NELF RNAi sample was treated with double-stranded RNAs
(dsRNAs) targeting NELF-B and NELF-E. The mock RNAi sample was treated with dsRNAs against
β-galactosidase. Treatment with the anionic detergent Sarkosyl results in the
resumption of transcription by paused polymerases.
The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the
accession number GSE23544.
The results were lifted from the D. melanogaster release 5 assembly to the release 6 assembly.
Core LJ, Waterfall JJ, Gilchrist DA, Fargo DC, Kwak H, Adelman K, Lis JT.
Defining the status of RNA polymerase at promoters.
Cell Rep. 2012 Oct 25;2(4):1025-35.