These tracks show the regions in the D. melanogaster genome that are significantly enriched in RNA Polymerase II (PolII) in different cell lines as determined by MACS2. The signal tracks correspond to the log likelihood enrichment ratios of the treatment versus the DNA input sample as determined by the
bdgcmp program in MACS2. The Irreproducible Discovery Rate (IDR) peak calls were produced by the idr program using a global IDR threshold of 0.05.
The RNA PolII ChIP-Seq reads for the S2 cells were obtained from the EBI ENA database under the accession number PRJEB3031. The antibody used in this study recognizes the Rpb3 subunit of RNA PolII.
Zhang Y, Liu T, Meyer CA, Eeckhoute J, Johnson DS, Bernstein BE, Nusbaum C, Myers RM, Brown M, Li W, Liu XS. Model-based analysis of ChIP-Seq (MACS).
Genome Biol. 2008;9(9):R137. doi: 10.1186/gb-2008-9-9-r137. Epub 2008 Sep 17.