This track shows the cumulative strand-specific RNA-Seq coverage for different developmental stages of D. melanogaster. The RNA-Seq read coverage for each sample was normalized by a wigsum of
100,000,000 (1 million 100bp reads) using the bam2wig.py program in RSeQC, and then combined into a single track.
The RNA-Seq reads were obtained from the
ENCODE data portal at UCSC on August 2019, and then mapped against
the D. melanogaster dm6 genome assembly using
Brown JB, et al.
Diversity and dynamics of the Drosophila transcriptome. Nature. 2014 Aug 28;512(7515):393-9.
Kim D, Langmead B, Salzberg SL.
HISAT: a fast spliced aligner with low memory requirements.
Nat Methods. 2015 Apr;12(4):357-60.
Wang L, Wang S, Li W. RSeQC: quality control of RNA-seq experiments. Bioinformatics. 2012 Aug 15;28(16):2184-5.