These tracks show the read coverage from Micrococcal Nuclease sequencing (MNase-seq) of untreated, mock treated, and NELF-RNAi treated Drosophila S2 cells.
The datasets were retrieved from the Gene Expression Omnibus database at NCBI under the
accession number GSE22119. The MNase-seq reads were mapped against the D. melanogaster genome using bowtie with the following parameters:
-m 1 -v 2 --fr.
The nucleosome positions and the smoothed coverage track were created using DANPOS2 with default parameters and
--paired 1. The Reads Per Genomic Content (RPGC) coverage track was created using deepTools with the following parameters:
--MNase --binSize 1 --normalizeTo1x 142000000.
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