>HindIII 2454 9575 14580 18519 -1 >EcoRI 917 1352 1800 1929 3407 3593 3805 4950 10380 10809 21514 -1 >EcoRV 926 2961 5729 7484 9140 18809 -1 >SacI 882 1187 3112 3997 5767 7433 9521 12356 -1