>HindIII 4398 5783 23598 23598 -1 >EcoRI 2238 2791 4161 9255 15652 -1 >EcoRV 1453 1485 1600 4347 4553 4908 5505 7049 -1 >SacI 1383 3467 3748 5190 6630 9694 10400 -1