>HindIII 2417 2799 2869 4981 8508 10939 -1 >EcoRI 1276 1551 1653 2581 9793 12562 -1 >EcoRV 2278 3599 4451 5589 7561 8137 -1 >SacI 2211 4276 12401 13437 -1