>HindIII 2422 2748 2880 4996 8557 10977 -1 >EcoRI 1276 1559 1658 2594 9803 12624 -1 >EcoRV 1649 2261 3584 4443 5564 7541 8057 -1 >SacI 2218 4280 12441 13294 -1