>HindIII 1211 1540 2173 2843 3585 4283 4655 8466 11399 -1 >EcoRI 2294 2908 3678 5167 8320 18611 -1 >EcoRV 2868 4002 4289 4366 6132 8447 10547 -1 >SacI 10279 33918 -1