>HindIII 4034 9975 27534 27534 -1 >EcoRI 1138 1286 1593 5470 14774 16570 -1 >EcoRV 4578 4905 5387 10939 -1 >SacI 1041 1106 1617 2899 4505 5455 7627 14723 -1