>EcoRV 2180 6418 6917 10770 24633 -1 >HindIII 1675 2037 4441 5029 7224 7627 10378 10877 -1 >EcoRI 1848 1892 1997 2886 3234 3452 3615 4345 5941 10264 -1 >SacI 440 4250 5189 5417 11928 23103 -1