>EcoRV 634 847 1351 2366 2485 2556 3094 4150 4801 5691 8860 -1 >HindIII 554 1653 2660 2882 3460 3516 3782 4565 11307 -1 >EcoRI 649 1110 1574 1980 2769 6060 6765 9610 15495 -1 >SacI 437 1480 3922 5069 13149 22625 24008 -1