>EcoRV 2245 3946 4088 4538 4957 8621 13646 -1 >HindIII 642 5260 6232 8140 10472 11566 -1 >EcoRI 2641 3377 3552 4158 8240 9704 10499 -1 >SacI 441 1354 1502 2246 2637 3399 3461 3895 5511 7095 10393 -1